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Figure 5 | Cell Communication and Signaling

Figure 5

From: B-cell translocation gene 2 mediates crosstalk between PI3K/Akt1 and NFκB pathways which enhances transcription of MnSOD by accelerating IκBα degradation in normal and cancer cells

Figure 5

BTG2-mediated MnSOD expression via crosstalk of Akt1 and NFκB in A549 cells. To further investigate the regulation of MnSOD expression by BTG2 via activations of Akt1 and NFκB pathways in addition to HeLa cells, A549 human lung cancer cells were subjected to transfection of BTG2/TIS21 and adenoviral transduction analyses for the following experiments. (A) RT-PCR analysis showing the increased expression of MnSOD gene in A549 cells after overexpression of BTG2. (B) Immunoblot analysis showing the degradation of IκBα after transcfection of BTG2 for 48 h. (C) ChIP assay revealing the interaction of p65 with κB-RE in the MnSOD gene in the BTG2 overexpressed cells. (D) Activation of NFκB pathway by Akt1 in the BTG2 transfected A549 cells. Degradation of IκBα in the BTG2 expresser was reduced by transfection of siAtk1 to A549 cells for 48 h.

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Cell Communication and Signaling

ISSN: 1478-811X