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Figure 1 | Cell Communication and Signaling

Figure 1

From: B-cell translocation gene 2 mediates crosstalk between PI3K/Akt1 and NFκB pathways which enhances transcription of MnSOD by accelerating IκBα degradation in normal and cancer cells

Figure 1

Upregulation of MnSOD expression in HeLa cells by BTG2/TIS21. (A) Transfection analysis; HeLa cells (2×105 cells in 60 mm) were transfected with pcDNA3-TIS21(HA) for 6 h along with the empty vector (pcDNA3-HA) to adjust total DNA contents, and then subjected to RT-PCR analyses using the primers described in the Additional file 5. Expression of MnSOD was increased in the BTG2 overexpressed samples in a concentration dependent manner. GAPDH was employed as a control expression of transcription. (B) Expression of MnSOD was increased along with the overexpression of BTG2/TIS21, examined by immunoblot analysis. Tubulin expression was used for loading control in each lane. (C) Transduction analysis; Ad-BTG2 was infected to HeLa cells (2 × 105 in 60 mm dish) for 5 h and then BTG2-mediated MnSOD expression was evaluated by RT-PCR. Expression of MnSOD was increased after Ad-BTG2 transduction. (D) To evaluate effect of endogenous BTG2/TIS21 gene on the expression of MnSOD, wild type-MEF and BTG2/TIS21 knockout-MEF lysates were subjected to immunoblot analysis. Endogenous level of MnSOD expression was lower in the TIS21−/−MEF than the wt-MEF, when measured by Image J software.

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Cell Communication and Signaling

ISSN: 1478-811X