Figure 7

Expression of ERK1/2 phosphorylation, c-fos transcription and cell cycle proteins in SSCs as well as in hepatic stem-like cells and mature hepatocyte-like cells derived from SSCs. (A) Immunocytochemistry revealed the expression of phosph-ERK1/2 in SSCs (Panel i), hepatic stem-like cells derived from SSCs (Panel ii), small hepatocytes derived from SSCs (Panel iii), and mature hepatocyte-like cells derived from SSCs (Panel iv). Scale bars in A = 50 μm. (B) Western blots showed the expression of ERK1/2 phosphorylation in SSCs (lane 1), hepatic stem-like cells derived from SSCs (lane 2), and mature hepatocyte-like cells derived from SSCs (lane 3). The expression of ERK2 was used as a loading control of total proteins. (C) RT-PCR revealed the transcription of c-fos mRNA in SSCs (lane 1), hepatic stem-like cells derived from SSCs (lane 2), and mature hepatocyte-like cells derived from SSCs (lane 3). Housekeeping gene Gapdh served as a loading control of total RNA. (D) RT-PCR displayed mRNA expression of cyclin A, cyclin B, cyclin D1, and cyclin E in SSCs (lane 1), hepatic stem-like cells derived from SSCs (lane 2), and mature hepatocyte-like cells derived from SSCs (lane 3). Gapdh was used as loading control of total RNA. (E) RT-PCR showed transcripts of CDK1 and CDK2 in SSCs (lane 1), hepatic stem-like cells derived from SSCs (lane 2), and mature hepatocyte-like cells derived from SSCs (lane 3). Gapdh served as loading control of total RNA. (F) Western blots revealed the expression of cyclin A, cyclin B, cyclin D 1, and cyclin E in SSCs (lane 1), hepatic stem-like cells derived from SSCs (lane 2), and mature hepatocyte-like cells derived from SSCs (lane 3). The expression of ACTB was used as a loading control of total proteins.