Figure 8
From: Hierarchical scaffolding of an ERK1/2 activation pathway
B-Raf and C-Raf interactions in A7r5 smooth muscle cells. (A) Interactions between Raf isoforms, IQGAP1 and caveolin-1 were assessed by immunoprecipitation experiments. Lysates were immunoprecipitated with an anti-GFP antibody as control, or with an anti-B-Raf or anti-C-Raf antibody. Co-immunoprecipitated B-Raf, C-Raf, caveolin-1 and IQGAP1 were detected by western blotting. (B) Statistical analysis of co-immunoprecipitated C-Raf, caveolin-1 and IQGAP1 from 7 independent IPs with the anti-B-Raf antibody (GFP shown as control). (C) Statistical analysis of co-immunoprecipitated B-Raf, caveolin-1 and IQGAP1 from 7 independent IPs with the anti-C-Raf antibody (GFP shown as control). Significance compared to control (*) and p values are indicated on graphs; error bars represent standard errors. n. s., not significant.