Figure 1
From: Hierarchical scaffolding of an ERK1/2 activation pathway
ERK1/2 subcellular localization does not show stimulus-specific differences. A7r5 cells were stimulated for 5 minutes with either a phorbol ester (DPBA) or with serum (FCS), or left unstimulated before subcellular fractionation by differential ultracentrifugation. Samples were analyzed by western blotting. (A) Average ERK1/2 subcellular distribution from four independent experiments is shown along with a typical western blot. (B) Control staining with marker proteins for the cytoskeletal fraction (caldesmon), the membrane fraction (integrin) and the cytosolic fraction (GAPDH). Please note that the integrin band appears as a doublet. Significance (*compared to unstimulated samples) and p-values are indicated on chart. n.s., not significant; error bars represent standard errors.