Figure 1
From: Cold shock Y-box protein-1 proteolysis autoregulates its transcriptional activities
Localization of YB-1 and deletion constructs of YB-1. A. Schematic overview on GFP-tagged YB-1 (deletion) constructs. B. Localization of WT YB-1-GFP and YB-1 deletion constructs in proliferating RMC. Cells were transfected with expression vectors encoding for WT YB-1-GFP, YB-1 deletion constructs, or GFP. The subcellular localization was determined by confocal laser scanning microscopy, cell architecture was visualized by PI-staining. In the right column a merged overlay of PI staining and GFP fluorescence is shown. The percentages of cells with GFP-staining only in the nucleus (N), predominantly in the nucleus and weak in the cytoplasm (NC), in both compartments equally (NC), predominantly in the cytoplasm (CN), and only in the cytoplasm (C) are provided. 100 transfected cells were assessed for each plasmid.