Figure 4

The α2-β4 region is important for Gα ₁₆ to interact with Fhit. A, Schematic representation of the N188, N210, N246, N266, C128 and C164 chimeras. Predicted secondary structures are illustrated as boxes (α helices) or ovals (β strands) above the chimeras. Sequences from human Gα_z are shaded in grey while those from human Gα₁₆ are in black. B, HEK293 cells were transiently co-transfected with Flag tagged Fhit and the wild-type or constitutively active mutants of Gα₁₆, Gα_z, N188, N210, N246, N266, C128 or C164. Cell lysates were immunoprecipitated with anti-Flag agarose affinity gel (upper panels). Expression levels of Gα₁₆, Gα_z, Flag-Fhit and α-tubulin in the total cell lysate were detected by western blotting (lower panels). Data shown represent one of three or more sets of immunoblots; other sets yielded similar results.