Figure 7
From: Phospholipase D regulates the size of skeletal muscle cells through the activation of mTOR signaling
PLD modulates the expression of atrogenes in myotubes. (A) Effects of adenovirus-mediated PLD overexpression on atrogene mRNA levels. GFP-adenovirus infected myotubes were used for control. (B) Effects of siRNA-mediated PLD depletion on the expression of atrogenes. An irrelevant siRNA was used for control. (C) Effects of PLD inhibition by FIPI on the expression of atrogenes. Myotubes were left untreated or were treated with 0.5 μM FIPI for 2 days. (D) Effect of the addition of 100 μM exogenous PA on the expression of atrogenes in the presence of 100 μM dexamethasone. Control cells received no treatment. All the mRNA levels were normalized to the levels of TBP mRNA in the samples. For all experiments, results are shown as means ± SE of n = 4 to 6 replicates. ***: different from control, p ≤ 0.001; **: p < 0.01; *: p < 0.05. +++: different from dexamethasone treated cells, p < 0.001.