Figure 4
From: Phospholipase D regulates the size of skeletal muscle cells through the activation of mTOR signaling
PLD1 overexpression in mice results in muscle hypertrophy. Adenovirus encoding PLD1 or GFP were respectively injected in the right and the left gastrocnemius of mice. (A) PLD1 overexpression was assessed by RT-qPCR of hPLD1 performed in both GFP- and PLD1-injected gastrocnemius muscles. Means ± SE of n = 5 are shown. ***: p < 0.0001. (B) Myofibre CSA measurements from transverse sections of gastrocnemius muscles injected with either GFP-adenovirus or PLD1-adenovirus. The mean CSA ± SE of 5 animals were compared by a paired t-test. **: p = 0.01. (C) Myofibre CSA distributions in GFP-adenovirus injected and PLD1-adenovirus injected muscles. Means ± SE of n = 5 are shown. **: p < 0.01; *: p < 0.05. (D) CSA of HA-PLD1 expressing fibres were compared to the CSA of non-PLD1 expressing fibres in sections of right gastrocnemius. **: p < 0.01 (means ± SE of 247 PLD1- and 149 PLD1+ counted fibres).