Figure 2
From: Phospholipase D regulates the size of skeletal muscle cells through the activation of mTOR signaling
Atrophic effects of PLD inhibitors on L6 myotubes. Differentiated L6 myotubes were cultured for 2 days without inhibitor (control), or in the presence of 0.5 μM FIPI, or 100 nM PLD1-inhibitor, or 100 nM PLD2-inhibitor. (A) Myotube area was measured as above. Data are means ± SE of n = 10. (B) Myotubes were homogenized and creatine kinase activity was measured. Data are means ± SE of n = 4. ***: different from control, p < 0.0001; **: p < 0.01.