Figure 1
From: Phospholipase D regulates the size of skeletal muscle cells through the activation of mTOR signaling
Atrophic effects of 1-butanol on L6 myotubes. Differentiated L6 myotubes were cultured for 2 days in the presence of 0.5% 1-butanol, or 0.5% 2-butanol, or left untreated (control). (A) Myotubes were immuno-stained with anti-MHC antibody, and myotube area was measured as reported in[30]. Data are means ± SE of n = 8. (B) Myotubes were homogenized and creatine kinase activity was measured. Data are means ± SE of n = 3. (C) MHC content of myotubes was assessed by ELISA. Data are means ± SE of n = 3. ***: different from control, p < 0.001, **: p < 0.01, *: p < 0.05.