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Figure 2 | Cell Communication and Signaling

Figure 2

From: Phosphodiesterase activity is regulated by CC2D1A that is implicated in non-syndromic intellectual disability

Figure 2

CC2D1A regulates PDE4D activity. A. Immunocytochemistry of forskolin induction time course 0, 10 and 15 minutes of wt and CC2D1A mutant (Δ) Mouse embryonic fibroblasts (MEF) co-stained with anti-CC2D1A and anti-PDE4D. The mouse mutant form (Δ) is similar to fragment VI since it contains only the first DM14 domain. B. Western blot of forskolin induction time course 0, 2, 7 and 30 minutes of wt and CC2D1A mutant (Δ) MEF cells probed with anti-CC2D1A (upper panel), anti- phospho PDE4D (middle panel) or anti-phospho CREB (lower panel)(n = 7). C. Western blot of forskolin induction time course 0, 2, 7 and 30 minutes of wt and CC2D1A mutant (Δ) MEF (shown in B) cells probed with anti-PDE4D (upper panel) or anti-CREB (lower panel). D. Endogenous PDE4 activity in forskolin stimulated wt and CC2D1A mutant (Δ) MEF cells. E. In vitro recombinant basal PDE4D5 (fragment IX) activity in the presence and absence of PKA and/or CC2D1A (I).

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