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Figure 4 | Cell Communication and Signaling

Figure 4

From: Asymmetric kinase dimer formation is crucial for the activation of oncogenic EGFRvIII but not for ERBB3 phosphorylation

Figure 4

Functional analysis of ERBB heterodimers. (A) Ba/F3 cells stably expressing indicated ERBB3 receptors were lysed and western blotting was performed with anti-ERBB3 and anti-beta-actin antibodies. Untransfected Ba/F3 cells were taken as a negative control. (B-E) Ba/F3 cell lines described above with or without stable expression of ERBB3 constructs were additionally infected with MSCV-GFP-EGFRvIII (B: i: control, ii: ERBB3-WT, iii: ERBB3-V945R), MSCV-GFP-EGFRvIII/V948R (C: i: control, ii: ERBB3-WT, iii: ERBB3-V945R), MSCV-YFP-EGFR (D: i: control, ii: ERBB3-WT, iii: ERBB3-V945R) or MSCV-GFP-ERBB2 (E: i: control, ii: ERBB3-WT, iii: ERBB3-V945R). Cells were then cultured with or without the indicated cytokines (IL-3, EGF (25 ng/ml) or heregulin (50 ng/ml). The Percentage of GFP-positive (EGFRvIII or ERBB2) or YFP (EGFR-WT)-positive cells was measured by FACS analysis at the indicated time points.

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Cell Communication and Signaling

ISSN: 1478-811X