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Figure 8 | Cell Communication and Signaling

Figure 8

From: Signal transduction in primary human T lymphocytes in altered gravity – results of the MASER-12 suborbital space flight mission

Figure 8

Acetyl-Histone H3 in T lymphocytes exposed to different gravity conditions measured by FACS-analysis. Non-activated and Concanavalin A (ConA)/CD28-activated CD4+ T lymphocytes were exposed to altered gravity. Samples were stained against acetyl-histone H3 and analyzed by FACS; data are expressed as relative fluorescence intensity (RFI). A: Analysis of non-activated (−) T cells revealed that microgravity (μg)-samples and on-board reference centrifuge (1g)-samples were stained significantly less for acetyl-histone H3 than hardware ground control (H/W)-samples. μg-samples and on-board reference centrifuge (1g)-samples did not differ in their staining intensities. B: In ConA/CD28 activated (+) T lymphocytes acetyl-histone H3-staining was neither significantly different between 1g and μg samples, nor between baseline (BL) samples and 1g or μg samples. BL samples were stained significantly less than H/W samples, and H/W samples were stained significantly stronger than culture control (CC) samples. The medians and individual interquartile ranges are shown. (* p < 0.1, ** p < 0.05, ns = not significantly different, two-tailed Mann–Whitney-U-Test).

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Cell Communication and Signaling

ISSN: 1478-811X