Figure 2

Morphology of HDFs stimulated with CM of M1 polarized, M2 polarized, or unstimulated macrophages. HDFs stimulated with CM of M1 polarized macrophages; 24 h (A), 72 h (D), and 144 h (G). After 24 h, most of the fibroblasts showed a spindle-shaped morphology although some rounded fibroblasts were seen. After 72 h, the fibroblasts adopted a rounded morphology that was most prominent after 144 h. HDFs stimulated with CM of M2 polarized macrophages; 24 h (B), 72 h (E) and 144 h (H). After 24 h the cells showed a spindle-like morphology, which was changed into an elongated spindle-like morphology after 72 h. HDFs stimulated with CM from unstimulated macrophages 24 h (C), 72 h (F), and 144 h (I). The fibroblasts showed a spindle-like morphology after 24 h, which was not changed in time.