Lnk recruits the intracellular part of InR to the membrane. CFP-tagged InR (fragments) are shown in ’ panels. Actin staining marks the cellular cortex in ” panels except for E and F where Lnk-RFP is shown in the ” panels. Merged pictures are shown in ”’ panels. Signal intensities measured along the indicated lines in ”’ panels are displayed in ”” panels. Quantifications in Additional file 1: Figure S1B. (A-B) InR-CFP is substantially reduced at the cellular cortex in lnk mutant tissue (B-B””) in comparison to wild-type salivary glands (A-A””). (C-C””) Localisation of InRINTRA-CFP (C’) in wild-type tissue. (D-D””) InRINTRA-CFP in lnk−/− tissue. (E-E””) Co-localisation of InRINTRA-CFP (E’) and Lnk-RFP (E”) at the cellular membrane. (F-F””) Co-localisation of InRINTRA-CFP (F’) and Lnk-PH*-RFP (F”) in the cytoplasm. Number of samples analysed: (A) n=5, (B) n=6, (C) n=6, (D) n=7, (E) n=6, (F) n = 5. Scale bars represent 50 μm.