Figure 2

All Gα subunits from the G _q subfamily can activate PKD1, PKD2 and PKD3. (A) Stably expressed and epitope-tagged PKD isoforms were immunoprecipitated from HEK293 cells transiently co-transfected with Gα subunits, and syntide-2 phosphorylation assays were carried out as described under “Materials and Methods”. Results are the average of at least three independent experiments, and presented as fold of control (±S.E.M.). (B) Cell lysates from HEK293 transfectants as described in (A) were subject to Western blot analysis using antibodies against specific Gα subunits (except anti-Gα_q which cross reacts with Gα₁₁), phosphorylated PKD isoforms, PKD1, 2, 3 as well as their corresponding tag. Similar results were obtained in three separate experiments.