Protein tyrosine phosphatase-1B regulates the tyrosine phosphorylation of the adapter Grb2-associated binder 1 (Gab1) in the retina

Table 1 Prediction of tyrosine phosphorylation on tyrosine residues in Gab1

Position of Tyr	Sequence¹	Score²	Prediction
24	KLKRYAWKR	0.050
47	DVLEYYKND	0.279
48	VLEYYKNDH	0.279
83	FENSYIFDI	0.687	“Y”
95	DRIFYLVAD	0.040
162	DPPPYQVIS	0.145
183	DPQDYLLLI	0.078
242	QQMMYDCPP	0.070
259	ESSLYNLPR	0.377
265	LPRSYSHDV	0.007
285	DGELYTFNT	0.896	“Y”
307	VSISYDIPP	0.487
317	PGNTYQIPR	0.498
373	TDSSYCIPP	0.909	“Y”
407	SQDCYDIPR	0.908	“Y”
428	FHSQYKIKS	0.419
448	LDENYVPMN	0.932	“Y”
473	QEPNYVPMT	0.980	“Y”
590	SEENYVPMN	0.991	“Y”
628	KQVEYLDLD	0.792	“Y”
660	ERVDYVVVD	0.961	“Y”

¹The amino acid sequence surrounding the Tyr (Y).
²Phosphorylation scores were calculated based on the phosphorylation site prediction program[42]. Scores above 0.5 are deemed to be possible phosphorylation sites and the higher the score, the more likely a particular site will be phosphorylated. Mus musculus Gab1 protein sequence was used for the analysis [accession number AJ 250669].

ISSN: 1478-811X