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Figure 9 | Cell Communication and Signaling

Figure 9

From: Protein tyrosine phosphatase-1B regulates the tyrosine phosphorylation of the adapter Grb2-associated binder 1 (Gab1) in the retina

Figure 9

Enhanced Gab1 tyrosine phosphorylation in PTP1B inhibitor-treated and PTP1B knockout mouse retinas. Retinal ex vivo explants from dark adapted rats were incubated with or without 1 μM insulin (5 min at RT) or DMSO (30 min at RT) or 100 μM PTP1B inhibitor (30 min at RT). Anti-IRβ and anti-Gab1 immunoprecipitates were immunoblotted with PY99 (A, B, C) and reprobed the blots with IRβ (A) or Gab1 (B, C) antibodies. Wild type, PTP1B and Akt2 knockout mouse retinal proteins were subjected to immunoblot analysis with anti-pGab1 and Gab1 (D, E) antibodies. The panel D, upper portion was stitched together from two areas of the blot representing the same migration window. Two independent mouse retinas were used in experiments described in Panel B, C, D and E.

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Cell Communication and Signaling

ISSN: 1478-811X