Figure 3
Light-dependent phosphorylation of Gab1 in the retina. Retina lysates from dark- and light-adapted rats were immunoprecipitated with Gab1 antibody followed by immunoblot analysis with anti-PY99 antibody (A). The blot was stripped and reprobed with anti-Gab1 antibody (A). Densitometric analysis of PY99 immunoblot was performed in the linear range of detection and absolute values were then normalized to Gab1 (B). Data are mean ± SD, n=4. H2O2-stimulated tyrosine phosphorylation of retinal Gab1. Retinal proteins from (two independent rats) controls and H2O2 (600 μM) treated ex vivo retinal explants were subjected to immuno blotting analysis with anti-pGab1, anti-Gab1 and anti-actin antibodies (C).