Figure 7
From: RACK1 is involved in endothelial barrier regulation via its two novel interacting partners
TIMAP interacts with farnesyl transferase via RACK1. (A) GST-RACK1 pull-down of endogenous farnesyl transferase (FNTA). GST, recombinant RACK1 WT or GST-RACK1 fragments were tested in pull-down assay. The immobilized samples were incubated with BPAEC lysate. Western blot of the pull-down eluates was probed with anti-FNTA antibody. (B) TIMAP or FNTA was immunoprecipitated from non transfected (ctr), non silencing RNA or siGBN2L1 transfected HPAEC. Total cell lysates and the IP complexes were probed for TIMAP and FNTA. Additional bands in IP samples correspond to IgG.