Figure 6
From: RACK1 is involved in endothelial barrier regulation via its two novel interacting partners
RACK1 depletion attenuates EC barrier function. Non transfected (ctr), non silencing or RACK1 specific silencing RNA transfected HPAEC were plated (5 x 105 cells/well) onto two 8W10E arrays 48 h post-transfection. (A): The initial resistance values at the beginning of the measurement were about 300 Ω and the impedance was measured for 20 hrs after the seeding. (B,C): After overnight incubation and basal TER monitoring (900–1200 Ω), HPAEC monolayers were treated with 50 μM forskolin (B) or 1 μM sphingosine 1-phosphate (S1P) (C). Relative resistances that were detected at the time of maximal TER increase of forskolin/S1P treated cells are shown for each sample. The results are presented as means ± SEM at least of four chambers for each treatment.