Insulin and insulin like growth factor II endocytosis and signaling via insulin receptor B

Table 1 Internalization of IGF-II and insulin through IR-B by microscopy

Time at 37°C	0 min		10 min		20 min		150 min
Ligand	Insulin	IGF-II	Insulin	IGF-II	Insulin	IGF-II	Insulin	IGF-II
Average	0.06	0.17	0.38	0.60	0.41	0.72	0.95	0.96
s.e.m	0.01	0.03	0.03	0.03	0.03	0.03	0.01	0.01
n	28	29	10	20	20	20	23	19
p (insulin versus IGF-II)	2E-03		9E-06		7E-09		9E-01

Quantification of the degree of internalization of IGF-II and insulin in HeLa cells expressing IR-B-SYFP labeled in vivo with 50 nM BAC-Ins and 1 nM QD655 at room temperature.

ISSN: 1478-811X