Figure 7
From: Cytosolic Ca2+ shifts as early markers of cytotoxicity
Caspase activation after sublethal and lethal doses of As 2 O 3 , gossypol, H 2 O 2 and staurosporine in HeLa and MCF-7 cells. (A) Western blot analyses of cleaved caspase 7 and 9 after staurosporine and As2O3 treatment in HeLa cells 4 h post treatment. Α-tubulin is shown as loading control. (B) Western blot analyses of cleaved caspase 7 and 9 after staurosporine and As2O3 treatment in MCF-7 cells 4 h post treatment. Α-tubulin and 1 μM staurosporine treated HeLa cells are shown as controls. (C) Western blot analyses of cleaved caspase 7 and 9 after gossypol treatment as indicated in HeLa and MCF-7 cells 4 h post treatment with α-tubulin as loading control. (D) Western blot analyses of cleaved caspase 7 and 9 after H2O2 treatment as indicated in HeLa and MCF-7 cells 4 h post treatment with α-tubulin as loading control. (E) Cell viability was assessed with Alamar Blue in HeLa cells in presence or absence of Q-VD-OPh (20 μM). Cells were challenged with EC75 values of As2O3, gossypol, H2O2 and staurosporine as indicated. Differences in cell survival of Q-VD-OPh plus toxin compared to toxin only treatment are shown (mean±SD; *p<0.05; n=7; t test).