Figure 3
From: Actin-dependent activation of serum response factor in T cells by the viral oncoprotein tip
Influence of dominant-negative GTPases on SRF activation. Jurkat T cells were transiently transfected with expression plasmids coding for myc-Rac1-T17N (RacT17N), RhoA-T19N (RhoT19N) and H-Ras-S17N (RasS17N) alone or in combination with a Tip expression construct. (A) Activity of cotransfected p3D.A-Luc displayed as mean values and standard deviation of three independent experiments. Statistical significance, p > 0.05 (ns); p < 0.01 (**). (B) Representative expression control carried out by immunoblot analysis. Detection of β-tubulin served as a loading control