Figure 4

Tor signaling input is not required for rapamycin inhibition of BVr enzyme. Sf9 cells were infected with S6Kα1WT, S6Kα1T412A and T252A (a) or S6Kα1WT & S6K ΔNH_2-46/ΔCT₁₀₄ (b) recombinant viruses at an M.O.I ≤ 1. Cells were exposed to rapamycin (50 nM) or vehicle, 24 h post infection for 15 min and harvested, lysed for IPs and subjected to S6 kinase as above. The figure is representative of three independent experiments.