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Figure 2 | Cell Communication and Signaling

Figure 2

From: Complex formation of EphB1/Nck/Caskin1 leads to tyrosine phosphorylation and structural changes of the Caskin1 SH3 domain

Figure 2

Expression of EphB1 in COS7 cells results in autoactivation and interaction with Nck. (a) COS7 cells were transiently transfected with HA-tagged EphB1 and after overnight serum-starvation cells were stimulated with ephrinB1 for 20 minutes or left untreated. Proteins were immunoprecipitated with anti-EphB1 antibody and probed with anti-phosphotyrosine and anti-HA antibodies. (b) HA-tagged EphB1 was transiently expressed in COS7 cells. Cell lysates were then subjected to immunoprecipitation with an anti-Nck antibody. Bound proteins were separated by SDS-PAGE, transferred to nitrocellulose, and probed with anti-phosphotyrosine, anti-HA, and anti-Nck antibodies. These results are representative of three experiments.

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Cell Communication and Signaling

ISSN: 1478-811X