Skip to main content
Figure 4 | Cell Communication and Signaling

Figure 4

From: Monocytes conditioned media stimulate fibronectin expression and spreading of inflammatory breast cancer cells in three-dimensional culture: A mechanism mediated by IL-8 signaling pathway

Figure 4

Expression of fibronectin by SUM149 cells. A) Immunoblot analysis revealed that fibronectin was weakly expressed by SUM149 cells grown in complete media (lane a) and highly expressed by SUM149 cells grown in U937-CM (lane b), β-actin was used as loading control. B) Immunostaining showed low expression of fibronectin (green) by SUM149 grown in complete culture media. C) SUM149 cells grown in U937-CM migrate and form branched like structures highly express cellular and membranous fibronectin. Nuclei were stained with DAPI (blue) and scale = 10 μm. Results were representatives of 3 independent experiments.

Back to article page

Cell Communication and Signaling

ISSN: 1478-811X