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Figure 3 | Cell Communication and Signaling

Figure 3

From: Monocytes conditioned media stimulate fibronectin expression and spreading of inflammatory breast cancer cells in three-dimensional culture: A mechanism mediated by IL-8 signaling pathway

Figure 3

Expression of E-cadherin by SUM149 cells. A) Immunoblot analysis did not detect any difference in E-cadherin expression by control SUM149 cells gown in complete culture media (lane a) versus those grown U937-CM (lane b), β-actin was used as loading control. B & C) Immunostaining showed no difference in expression of E-cadherin (red) by SUM149 cells grown in complete culture media (B) versus SUM149 cells grown in U937-CM (C). While, SUM149 cells grown in U937-CM (C) migrate and form branched like structures. Nuclei were stained with DAPI (blue) and scale bar = 10 μm. Results were representatives of 3 independent experiments.

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