InsR silencing induced reduction in cellular accumulation of FN was partially dependent on PI3K/Akt. Cells were transfected with SC, sh-InsR, sh-InsR + dominant negative Akt (DN-Akt) cDNA or sh-InsR + activated H-Ras (aH-Ras) cDNA. Cell lysates were subject to Western blotting with an anti-FN antibody. A gel stained after transfer was shown as a loading monitor. The bar graph shows the densitometric scanning result (means ± SEM) from four independent experiments. *, P<0.01, NS, no significant difference.