Figure 5

ARTD10 forms dynamic bodies in cells. A. HeLa cells were fixed and stained with an ARTD10-specific antibody (E09). The DNA was labeled with Hoechst 33258. B. CFP-YFP-ARTD10 was expressed in COS7 cells. A frame with 4 cells was analyzed using live-cell imaging for 256 minutes with one picture taken every 4 minutes. Arrows indicate ARTD10 bodies that fuse, the arrowheads a body that moves within the cell, and double-arrows bodies that appear and disappear. C. A single ARTD10 body was bleached at high laser intensity (upper panel). The recovery of the fluorescence signal was monitored by live-cell imaging. Using the mean of 12 bleaching experiments in different cells the FRAP kinetics were quantified and are displayed with standard deviations (lower panel).