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Figure 4 | Cell Communication and Signaling

Figure 4

From: EGF and HB-EGF enhance the proliferation of programmable cells of monocytic origin (PCMO) through activation of MEK/ERK signaling and improve differentiation of PCMO-derived hepatocyte-like cells

Figure 4

MEK/ERK signaling drives proliferation in PCMOs and is superactivated by EGF and HB-EGF. (A) M-CSF and Il-3 induce phosphorylation of ERK (pERK). Protein samples were obtained from PCMOs cultured for various days (as indicated) in standard PCMO differentiation medium. (B) Inhibition of ERK was sufficient to inhibit PCMO proliferation. Cells were cultured with either solvent (left) or the ERK inhibitor U0126 (right) for 6 days and images were taken. Original magnification: 50x. (C) HB-EGF and EGF induce phosphorylation of MEK and ERK. Phospho-MEK (p-MEK), MEK1/2, phospho-ERK (p-ERK), and ERK1/2 expression was examined by immunoblotting in lysates of day-4 PCMOs that have been treated with the indicated concentrations of either HB-EGF or EGF.

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Cell Communication and Signaling

ISSN: 1478-811X