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Table 2 Transcript levels of macrophage genes involved in lipid metabolism altered by Mtb infection

From: Strain specific transcriptional response in Mycobacterium tuberculosis infected macrophages

Gene Annotation Expression Ratio (HN878/CDC1551) Function
Array §
Scd1 Steroyl-CoA desaturase 1 1.85 12.13* Fatty acid synthesis
Fads2 Fatty acid desaturase 2 2.95 8.51* Lipid metabolism
Gpd2 Glycerol-3-phosphate dehydrogenase 1.35 3.61* Glycerolphospholipid metabolism
Adora2b Adenosine A2b receptor 1.63 3.59* Lipid synthesis
Fdft1 Farnesyl diphosphate fanesyltransferase 1 1.53 2.77* Cholesterol synthesis
Gbgt1 Globoside alpha-1,3-N-acetogalatosaminyltransferase 1 1.64 2.75* Glycolipid metabolism
Acsl1 Acyl-CoA synthetase long chain family member 1 1.71 2.69* Fatty acid synthesis
Acss2 Acyl-CoA synthetase short chain family member 1 1.53 2.58* Lipid synthesis
Hsd17b7 Hydroxysteroid 17-beta dehydrogenase 7 1.39 2.58 prostaglandin metabolism
Lss1 Lanosterol synthase 1 1.34 2.51* Fatty acid metabolism
Dhcr24 24-dehydrocholestrol reductase 2.30 2.36* Cholesterol synthesis
Ppap2b Phosphatidic acid phosphatase type 2B 1.35 2.26* Lipid synthesis
Acat2 Acyl-CoA thioesterase 7 1.43 1.82* Fatty acid metabolism
Scd2 Steroyl-CoA desaturase 2 1.63 1.80 Fatty acid synthesis
Mvd Mevalonate decarboxylase 1.42 1.72* Cholesterol synthesis
Fdps Farnesyl diphosphate synthase 1.30 1.64 Sterol synthesis
Ptges Prostaglandin E synthase 1.92 1.60* Prostaglandin metabolism
Cyp51 Cytochrome P450, family 51, subfamily A, polypeptide 1 1.50 1.35* Sterol synthesis
Idi1 Isopentenyl diphosphate delta isomerase 1 1.69 ND Cholesterol synthesis
  1. § values are averages compiled from at least 3 independent experiments with each Mtb strain.
  2. values are averages compiled from at least 3 independent experiments and differentially expressed macrophage genes between CDC1551 and HN878 infection that are statistically significant by both microarray and qRT-PCR are marked with an asterisk (*).
  3. Significantly differentially expressed macrophage genes involved in lipid metabolism at 24 hpi by Mtb CDC1551 or HN878. Levels of gene expression were derived from microarray and qRT-PCR experiments. Relative gene expression ratio for microarray and qRT-PCR was determined by dividing the expression values from HN878-infected BMM by that of CDC1551-infected BMM. ND- transcript not detected.