Figure 3
From: Strain specific transcriptional response in Mycobacterium tuberculosis infected macrophages
Expression of cytokine, chemokine and inflammatory genes during Mtb infection of BMM. Total RNA from BMM infected with CDC1551 or HN878 at 6 and 24 hpi was used to quantify the transcript levels of cytokines (Il1β and Il6), chemokines (Cxcl9 and Ccl8), and inflammatory molecules (Mmp9 and Cd209g) by qRT-PCR. The threshold cycle (Ct) of each test gene was normalized against that of the house-keeping gene, Gapdh and the relative fold change in gene expression was calculated by comparing Mtb-infected and uninfected samples. The data presented are average ± standard deviation of values obtained from three independent infections per experimental group assayed at least in duplicate. * P ≤ 0.05.