Expression of cytokine, chemokine and inflammatory genes during Mtb infection of BMM. Total RNA from BMM infected with CDC1551 or HN878 at 6 and 24 hpi was used to quantify the transcript levels of cytokines (Il1β and Il6), chemokines (Cxcl9 and Ccl8), and inflammatory molecules (Mmp9 and Cd209g) by qRT-PCR. The threshold cycle (Ct) of each test gene was normalized against that of the house-keeping gene, Gapdh and the relative fold change in gene expression was calculated by comparing Mtb-infected and uninfected samples. The data presented are average ± standard deviation of values obtained from three independent infections per experimental group assayed at least in duplicate. * P ≤ 0.05.