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Figure 2 | Cell Communication and Signaling

Figure 2

From: Expression of protein-tyrosine phosphatases in Acute Myeloid Leukemia cells: FLT3 ITD sustains high levels of DUSP6 expression

Figure 2

FLT3 ITD expressing cells exhibit high DUSP6 protein expression. Lysates of different cell lines (as indicated) were subjected to SDS-PAGE and immunoblotting to evaluate DUSP6 protein levels. For loading control, levels of vinculin and β-actin were also detected, and FLT3 levels were assessed for comparison. (A) RS4-11 cells, endogenously expressing wildtype FLT3, were compared with MV4-11 cells, endogenously expressing FLT3 ITD. Numbers under the lanes represent mean values (± SD) for the quantification of blots of 3 separate experiments (RS4-11 set to 1.0). (B) 32D cell lines either mock-transfected (“parental”), or stably transfected with wildtype FLT3 (WT) or FLT3 ITD (as indicated) were compared. Numbers represent quantification of 4 separate experiments (DUSP6 signal normalized to actin or vinculin; mean ± SD, parental set to 1.0; statistic significance was tested by Students’ test (*p < 0.05, **p < 0.01).

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Cell Communication and Signaling

ISSN: 1478-811X