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Fig. 2 | Cell Communication and Signaling

Fig. 2

From: High glucose-induced p66Shc mitochondrial translocation regulates autophagy initiation and autophagosome formation in syncytiotrophoblast and extravillous trophoblast

Fig. 2

p66Shc promotes autophagy in trophoblast cells. A Schematic structure representations of three isoforms for p46Shc, p52Shc and p66Shc, and the locations of phosphorylatable serine at position 36 in the CH2 domain and two glutamic acid residues at positions 132 and 133 in the CB domain. B-C qRT-PCR analysis of p66Shc mRNA expression B and Western blot images of p66Shc protein expression C in p66WT, p66SC and p66QQ cells. *P < 0.05, **P < 0.01. D Western blot images of protein levels of p66Shc, ATG5 and LC3 in p66WT, p66SC and p66QQ overexpressed HTR8/SVneo cells treated with Chloroquine. E Representative confocal images of autophagic flux were obtained from HTR8/SVneo cells overexpressing p66WT, p66SC, and p66QQ and transfected with mCherry-EGFP-LC3 to label the autophagosomes (yellow) and autolysosomes (cherry). Scale bar, 10 μm

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Cell Communication and Signaling

ISSN: 1478-811X