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Fig. 1 | Cell Communication and Signaling

Fig. 1

From: High glucose-induced p66Shc mitochondrial translocation regulates autophagy initiation and autophagosome formation in syncytiotrophoblast and extravillous trophoblast

Fig. 1

High glucose increased expression of p66Shc and its translocation to mitochondrion. A Western blot images and quantifications of protein expressions of p-p66Shc in cytosol, p66Shc in mitochondria and whole cell lysate in NP and GDM placentae. *P < 0.05, **P < 0.01. B Western blot images and quantifications of protein expressions of p-p66Shc in cytosol, p66Shc in mitochondria and whole cell lysate in 5 mM or 30 mM glucose treated HTR8/SVneo cells. *P < 0.05, **P < 0.01. C Fluorescence microscope images of co-localization of p66Shc (green) with mitochondria (red) in 5 mM or 30 mM glucose treated HTR8/SVneo cells. Scale bar, 20 μm. D Box plot shows the levels of maternal serum Mn-SOD between NP (n = 281) and GDM patients (n = 1037). E Gene ontology analysis of differentially expressed genes between NP and GDM placentae. F MitoSOX staining showing levels of mitochondrial superoxide in 5 mM or 30 mM glucose treated HTR8/SVneo cells. Scale bar, 50 μm

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Cell Communication and Signaling

ISSN: 1478-811X