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Fig. 6 | Cell Communication and Signaling

Fig. 6

From: Exploring the role of Prx II in mitigating endoplasmic reticulum stress and mitochondrial dysfunction in neurodegeneration

Fig. 6

Prx II protects alcohol-induced decrease in mitochondrial membrane potential by scavenging ROS. (A) Detection of intracellular ROS levels in HT22 cells after alcohol treatment using fluorography. (B) Detection of the PERK signaling pathway after NAC pretreatment using western blotting. (C) Detection of ATF3 protein expression levels after NAC pretreatment using western blotting. (D) Detection of ATF3 mRNA expression levels after NAC pretreatment using qRT-PCR. (E) Detection of miR-181b-5p expression level after NAC pretreatment using qRT-PCR. (F) Detection of Armcx3 protein expression level after NAC pretreatment using western blotting. (G) Detection of Armcx3 mRNA expression level after NAC pretreatment using qRT-PCR. (H) Detection of Ca2+ level in mitochondria after NAC pretreatment using flow cytometry. (I) Quantitative analysis of the data in panel “h.” (J) Detection of mitochondrial membrane potential after NAC pretreatment using fluorography. Each analysis was performed in triplicate

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Cell Communication and Signaling

ISSN: 1478-811X