Fig. 5

Re-transfer of Prx II gene to identify the mechanism through which Prx II protects alcohol-induced decrease in mitochondrial membrane potential. (A) Flow cytometric detection of intra-mitochondrial Ca²⁺ levels. (B) Quantitative analysis of the data in panel “A.” (C) Fluorographic detection of mitochondrial membrane potential. (D) Western blotting was used to detect PERK signaling pathway protein expression levels. (E) ATF3 mRNA expression levels were detected using qRT-PCR. (F) Western blotting was used to detect ATF3 protein expression levels. (G) The expression levels of miR-181b-5p were detected using qRT-PCR. (H) Armcx3 mRNA expression levels were detected using qRT-PCR. (I) Western blotting was used to detect Armcx3 protein expression levels. Each analysis was performed in triplicate