Fig. 4

Prx II regulates Armcx3 expression by miR-181b-5p, through the transcription factor ATF3. (A) The miRWalk, miRDB, and starBase databases predicted the miRNAs that target Armcx3, which were plotted in a Venn diagram with reciprocal intersection. (B, C) The expression levels of miRNAs predicted by the miRWalk, miRDB, and starBase databases in mock HT22 and shPrx II HT22 cells following alcohol treatment, were detected using qRT-PCR. (D) ATF3 mRNA expression levels were detected using qRT-PCR. (E) Western blotting was used to detect ATF3 protein expression levels. (F) ATF3 mRNA expression after the addition of SC79 was detected using qRT-PCR. (G) ATF3 mRNA expression after the addition of 4PBA was detected using qRT-PCR. (H) The expression levels of miR-181b-5p after 4PBA pretreatment were detected using qRT-PCR. (I) Western blotting was used to detect Armcx3 protein expression levels after 4PBA pretreatment. Each analysis was performed in triplicate