Fig. 1

Prx II regulation of EMCS formation in HT22 inhibits alcohol-induced decrease in mitochondrial membrane potential. (A) Analysis of EMCS formation using transmission electron microscopy, with the ER labeled as ER and mitochondria labeled as M. The distance between the ER and mitochondria is marked with red dashed lines. (B) Quantification of the length of EMCS. (C) The percentage of mitochondria in contact with ER. These images are representative of three independent experiments, each with n = 3 technical replicates. (D) Flow cytometry detection of intra-mitochondrial Ca²⁺ levels; (E) Quantitative analysis of the data in panel “d.” (F) Western blotting detection of mitochondria-dependent apoptosis-related protein expression levels after BAPTA pretreatment. (G) Cytometry detection of intra-mitochondrial Ca²⁺ levels after BAPTA pretreatment. (H) Quantitative analysis of the data in panel “g.” (I) Fluorographic detection of mitochondrial membrane potential after BAPTA pretreatment. (J) MTT assay detection of BAPTA pretreatment after alcohol-induced HT22 cell death