Fig. 7
From: Structural determinants for activation of the Tau kinase CDK5 by the serotonin receptor 5-HT7R
Mutations of F278A and F281A ameliorates 5-HT7R-induced Tau pathology. A, B Phosphorylation levels of endogenous CDK5 in N1E-115 expressing HA-tagged 5-HT7R WT or indicated phenylalanine mutants. Representative Western blot (A) and quantification (B). Data is represented as mean ± SD (N = 4, one-way ANOVA, Dunnett’s multiple comparisons, * p < 0.05). C, D. N1E-115 cells were transfected with eGFP-Tau[R406W] mutant, together with indicated HA-tagged 5-HT7R constructs. Phospho-Tau and total Tau levels were detected with AT270 and 5A6 antibodies, respectively. Resulting ratios (D) were normalized to GAPDH expression and are shown as normalized mean ± SD (N = 3, one-way ANOVA, Dunnett’s multiple comparisons, * p < 0.05). E The number of Tau aggregate-positive cells was counted in a confined area and is presented as a fraction of the total number of transfected cells. Data is presented as normalized mean ± SD (N = 3, n ≥ 353, Dunnett’s multiple comparisons, ** p < 0.01, *** p < 0.001)