Fig. 6
From: Structural determinants for activation of the Tau kinase CDK5 by the serotonin receptor 5-HT7R
Mutations of F278A and F281A impairs interaction of 5-HT7R with CDK5. A, B N1E-115 cells were co-transfected with mCherry-tagged CDK5 and HA-tagged 5-HT7R constructs as indicated, followed by IP with anti-mCherry antibody and Western blot with anti-HA antibody. Quantification of the co-IP experiments is shown on the right. Ratios of co-precipitated receptors was calculated, normalized to the WT sample and are presented as mean ± SD (N = 3, one-way ANOVA, Dunnett’s multiple comparisons, * p < 0.05). C Representative confocal images of N1E-115 cells co-expressing either CDK5-eCFP and eYFP-5-HT7R mutants F278A, F281A or F278A; F281A. Scale bar: 10 μm. Corresponding intensity profiles are shown on the right. See also Additional file 13