Fig. 1
From: Structural determinants for activation of the Tau kinase CDK5 by the serotonin receptor 5-HT7R
5-HT7R and CDK5 interact and are co-localized at the plasma membrane. A Representative confocal images showing N1E-115 cells co-expressing either CDK5-eCFP and eYFP (control, upper panel) or CDK5-eCFP and 5-HT7R-eYFP (lower panel). Corresponding intensity profiles are shown below. Scale bar: 10 μm. See also Additional file 4. B N1E-115 cells were co-transfected with eCFP-tagged CDK5 and mCherry-tagged 5-HT7R, followed by immunoprecipitation (IP) with anti-mCherry antibody and Western blot with anti-CFP antibody. As a control, mixed lysates from the single transfected cells (mix) were applied to the IP