Fig. 7

The inflammatory state of patients with rheumatic joint diseases affects monocyte reaction to Wnt-3a stimulus. A Principal component analyses (PCA) based on RT-qPCR data (presented in Fig. S6B) of Wnt-3a-treated monocytes (treated as in Fig. 4E). The blue X represents the centroid of the healthy donor samples, calculated as the average of the x and y coordinates of all the blue dots on the PCA graph. The bar graphs represent a calculation of distance of each dot from the healthy donor centroid, based on x,y coordinates. One-way ANOVA with Dunnett's multiple comparisons test to healthy control were employed. Healthy donors (blue), treated RJD patients (pink) and RJD patients naïve to treatment (maroon). **P-value = 0.0024, ns = 0.6717. B Media were collected from the treated monocytes of two RJD patients naïve to immunosuppressive treatment. The media were subjected to a chemokine membrane array and analyzed, as performed with media of healthy donors in Fig. 5. The heatmap was created using numerical data of both the data of healthy donors represented in Fig. 5 (Healthy 1&2) and the data of the two RJD patients (RJD 1&2). The fold change (FC) of signal ratio of Wnt-treated to control-treated monocyte is shown as a heatmap (log2(FC)), where upregulated genes are represented in red, downregulated in blue, and unchanged in white. Hierarchical clustering was performed on both the donors on the X-axis and the secreted proteins on the Y-axis. Only proteins clearly detected across all four membranes are represented. Original images and analyses of all 4 donors are presented in Fig. S4, Fig. S7, and the raw figure data (Supplementary file 3). RJD – rheumatic joint diseases