Fig. 2

Wnt-3a induces expression and nuclear accumulation of β-catenin in monocytes. A and B, THP-1 cells (monocyte-like cells), PMA-treated THP-1 cells (macrophage-like cells), and freshly isolated primary monocytes were cultured for 5 h in the presence of control or Wnt-3a conditioned media (CM). A Cells were lysed, and western blot analysis was conducted using specific antibodies as indicated (representative blot). B Quantification of band intensity of experiments as in A, calculated by the ImageJ software. The Y-axis represents the band intensity of β-catenin standardized to actin (β-catenin/actin) of Wnt-treated cells relative to control-treated cells (100%). C-E Treated monocytes (as in A) were stained and visualized using immunofluorescence. Images were obtained using a confocal microscope with a 60X oil-immersion objective lens C. An independent script was used to quantify the signal intensity and nuclear localization of β-catenin. Pairs signify individual donors, where each point represents the mean value of at least 5 fields D, E (see methods section). *P-value = 0.0359 for paired t-test of signal intensity D and *P-value = 0.0342 for paired t-test of nuclear localization E