Fig. 1

Human monocytes express Wnt signaling components and the Wnt-3a ligand is found in the plasma. A Analysis of single-cell RNA-seq data by 10X genomics, based on a Seurat tutorial (“pbmc3k”). tSNE plot of the data with annotation of cell types (upper left panel). Classical monocytes are the orange cluster labeled “CD14⁺ Mono” and non-classical monocytes are the light blue cluster labeled “CD16⁺ Mono”. Plt – platelets. NK – natural killer cells. B and T lymphocytes are abbreviated B and T, respectively. The monocyte cluster (red circle) was further analyzed for expression of Wnt signaling components. The genes are grouped by functional categories (color-coded at the bottom). B Wnt-3a levels were measured by ELISA in isolated human plasma obtained from multiple healthy donors. Protein concentrations were calculated by regression from a standard curve