Fig. 7

Depletion of EMCN reduces the phosphorylation of FAK and Src following VEGFR2 activation. A Western blot image of p-VEGFR2, total VEGFR2, p-Src, total Src, p-FAK, total FAK, p-ERK1/2, total ERK1/2, and tubulin probing for a VEGF121 stimulation time course with and without EMCN. An equal quantity of protein was loaded into each well. B Densitometric analysis of western blot data demonstrated that knockdown of EMCN did not alter VEGF121-induced VEGFR2 activation. n = 3. C Western blot analysis demonstrated that VEGF121 activation of FAK at 10 min. D and Src at 10 min was diminished E whereas ERK activation was not affected by EMCN knockdown. One-way ANOVA analysis was used for comparation between different time points within group. Student-t test was used for comparation between siNT and siEMCN at the same time point. Statistical analysis. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001 n ≥ 3