Fig. 2

AP2 adaptor complex is essential for VEGFR2 CME following VEGF165 stimulation. A Internalized VEGFR2 following VEGF stimulation or BSA control was visualized by intracellular fluorescence intensity (red). Clathrin HC (heavy chain) (white) was co-visualized in each condition. In the absence of AP2A2 subunit, VEGF-induced VEGFR2 internalization (red) was reduced after 30 min. Bar = 20 µm B VEGFR2 internalization was quantified by relative fluorescence intensity and normalized to the total cell number per viewing field. Student-t test was used for statistical analysis. *P < 0.05, n = 6. C HRECs incubated in serum-free media were stimulated with VEGF165 (10 ng/ml) for 30 min with and without AP2A2 subunit knockdown, and cell surface VEGFR2 levels were analyzed by western blot. D Quantification of cell surface VEGFR2 band intensity normalized to CD31. One-way ANOVA was used for statistical analysis. ***P < 0.001, n = 3