Fig. 1

Potential binding proteins of EMCN involved in endocytosis identified through mass spectrometry. A Diagram illustrating the workflow of EMCN immunoprecipitation in HRECs lysate and subsequent identification of potential EMCN-binding proteins using mass spectrometry. B Coomassie blue stained SDS-PAGE gel showing the separation of EMCN binding proteins, which were then excised for mass spectrometric analysis. C Protein clusters involved in endocytosis among EMCN-binding proteins identified by mass spectrometry and analyzed using the STRING database. D List of endocytosis related EMCN binding proteins identified in mass spectrometry. E Validation of the interaction between EMCN and AP2A2 by immunoprecipitation on Western blot. F HRECs overexpressing myc-tagged EMCN were lysed and both AP2 α and β subunits co-immunoprecipitated with EMCN. FT: flowthrough. n = 3